Eileen K. Bridge
Associate Professor of Microbiology
Adenovirus (Ad) is a linear double-stranded DNA virus. Successful lytic infection by Ad requires a productive interaction with its host cell, creating an environment that is conducive to efficient viral gene expression, replication, and assembly of new progeny virus. Ad regulatory proteins derived from early region 4 (E4), E4-11K and E4-34K, and an early region 1b protein, E1b-55K, are important in cell transformation, viral replication, and late gene expression; they are also able to interact with host double-strand break repair (DSBR) proteins and affect their localization and stability. E4 mutants have their genomes "repaired" in infected cells to form concatemers of several genome lengths. My group is investigating the role of host DSBR processes in the replication and late gene expression defects displayed by E4 mutants lacking E4-11K and E4-34K. We are studying the relationship between the function of E4 proteins in inactivating DSBR, and their role in promoting viral replication and gene expression. We are investigating the role of genome concatenation for viral DNA replication and late gene expression in E4 mutant infections. We are also studying the interactions of host DSBR proteins with viral replication foci and viral DNA in E4 mutant infections. These studies are expected to further our understanding of how viral genes affect host cell activities, and how they may ultimately affect cell growth and transformation as they reprogram the machinery of the host cell to promote a successful lytic viral infection.
- Mathew, S.S., and E. Bridge. 2007. The cellular Mre11 protein interferes with adenovirus E4 mutant DNA replication. Virology. 365:346-55
- Jayaram, S., and E. Bridge. 2005. Genome concatenation contributes to the late gene expression defect of an adenovirus E4 mutant. Virol. 342:286-296.
- Corbin-Lickfett, K., and E. Bridge. 2003. Adenovirus E4-34kDa requires active proteasomes to promote late gene expression. Virol. 315:234-244.
- Carter, C.C., R. Izadpanah, and E. Bridge. 2003. Evaluating the role of CRM1-mediated export for adenovirus gene expression. Virol., 315:224-233.
- Bridge, E., K. Mattsson, A. Aspegren, and A. Sengupta. 2003. Adenovirus early region 4 promotes the localization of splicing factors and viral RNA in late phase interchromatin granule clusters. Virol. 311:40-50.
- Aspegren, A., and E. Bridge. 2002. Release of sn RNP and RNA from transcription sites in adenovirus-infected cells. Exp. Cell Res. 276:273-283.